@article {26445,
	title = {On-site Produced Enzyme Cocktails for Saccharification and Ethanol Production from Sugarcane Bagasse Fractionated by Hydrothermal and Alkaline Pretreatments},
	journal = {Waste and Biomass Valorization},
	volume = {13},
	year = {2021},
	pages = {95-106},
	abstract = {Enzymatic blends produced by fungal monocultures and consortia cultured in solid-state fermentation (SSF), using sugarcane bagasse (SB) and wheat bran as substrates (1:1, w/w), were evaluated for saccharification of sugarcane bagasse pretreated by autohydrolysis (hydrothermal pretreatment{\textemdash}HP) and alkaline delignification (HP-Soda). The highest glucose releases were obtained after saccharifications of SB pretreated by HP using enzyme cocktails produced by\&nbsp;Aspergillus niger\&nbsp;and by the consortium among\&nbsp;A. fumigatus,\&nbsp;Ganoderma lucidum\&nbsp;and\&nbsp;Trametes versicolor, with 10.8 and 9.8\&nbsp;g L-1, respectively. For SB pretreated by HP-Soda, the hydrolysate 10 (extract from\&nbsp;A. niger, G. lucidum\&nbsp;and\&nbsp;Pleurotus ostreatus\&nbsp;consortium) achieved maximal glucose concentration (11.92\&nbsp;g L-1). After alcoholic fermentation of the hydrolysates, the greatest ethanol yield in relation to the maximum theoretical yield (60.8\%) was obtained in the fermentation of hydrolysate 1 (A. niger) obtained from SB pretreated by HP-Soda. These results demonstrated that on-site produced enzyme cocktails can be applied for saccharification of pretreated sugarcane bagasse and also contribute to cost reduction of bioconversion processes.},
	url = {https://doi.org/10.1007/s12649-021-01499-7},
	author = {Patr{\'\i}sia de Oliveira Rodrigues and Elisa da Silva Barreto and Rog{\'e}lio Lopes Brand{\~a}o and Leandro Vin{\'\i}cius Alves Gurgel and Daniel Pasquini and Milla Alves Baffi}
}
