@article {26700,
	title = {Genetic evaluation of migratory fish: Implications for conservation and stocking programs},
	journal = {Ecology and Evolution},
	volume = {10},
	number = {16},
	year = {2020},
	abstract = {
	Fish stocking programs have been implemented to mitigate the blockage of original riverbeds by the construction of hydropower dams, which affects the natural migration of fish populations. However, this method raises concerns regarding the genetic rescue of the original populations of migratory fish species. We investigated the spatial distribution of genetic properties, such as genetic diversity, population structure, and gene flow (migration), of the Neotropical migratory fish\&nbsp;Prochilodus costatus\&nbsp;in the Tr{\^e}s Marias dam in the S{\~a}o Francisco River basin, Brazil, and examined the possible effects of fish stocking programs on\&nbsp;P.\&nbsp;costatus\&nbsp;populations in this region. In total, 1,017 specimens were sampled from 12 natural sites and a fish stocking program, and genotyped for high-throughput sequencing at 8 microsatellite loci. The populations presented low genetic variability, with evidence of inbreeding and the presence of only four genetic pools; three pools were observed throughout the study region, and the fourth was exclusive to one area in the Paraopeba River. Additionally, we identified high unidirectional gene flow between regions, and a preferred migratory route between the Par{\'a} River and the upper portion of the S{\~a}o Francisco River. The fish stocking program succeeded in transposing the genetic pools from downstream to upstream of the Tr{\^e}s Marias dam, but, regrettably, promoted genetic homogenization in the upper S{\~a}o Francisco River basin. Moreover, the data show the fragility of this species at the genetic level. This monitoring strategy could be a model for the development of conservation and management measures for migratory fish populations that are consumed by humans.

},
	url = {https://doi.org/10.1002/ece3.6231},
	author = {Juliana da Silva Martins Pimentel and Sandra Ludwig and Leonardo Cardoso Resende and Pedro Ferreira Pinto Brand{\~a}o-Dias and Adriana Helo{\'\i}sa Pereira and Nazar{\'e} L{\'u}cio de Abreu and Izinara Cruz Rosse and et. al.}
}
