@mastersthesis {26446,
	title = {Estudos sobre consumo de glicerol por leveduras para fins biotecnol{\'o}gicos},
	year = {2019},
	type = {Tese de Doutorado},
	abstract = {Nos {\'u}ltimos anos o aumento do excedente de produ{\c c}{\~a}o do glicerol bruto, um subproduto da produ{\c c}{\~a}o do biodiesel, tem estimulado a busca por processos alternativos de aplica{\c c}{\~a}o do glicerol. De entre as alternativas, destacam-se os processos biotecnol{\'o}gicos onde o glicerol {\'e} convertido por microrganismos em um produto com maior valor agregado, de amplo uso industrial e com menor impacto ambiental. O objetivo do presente trabalho foi caracterizar Wickerhamomyces anomalus LBCM1105, uma cepa capaz de utilizar eficientemente o glicerol como {\'u}nica fonte de carbono, atrav{\'e}s do isolamento e caracteriza{\c c}{\~a}o do transportador de alta afinidade de glicerol (WaStl1p), e do sequenciamento do seu genoma. Estrat{\'e}gias de engenharia gen{\'e}tica foram tamb{\'e}m utilizadas em Saccharomyces cerevisiae BY4741, com intuito de otimizar o consumo de glicerol e obter a sua convers{\~a}o em etanol. LBCM1105 apresentou velocidades de crescimento maiores que BY4741 independente do meio (YP ou YNB) e da fonte de carbono (glicose ou glicerol). Com o objetivo de melhorar o desempenho de BY4741 em glicerol, foi tentada a sobrexpress{\~a}o simult{\^a}nea do transportador ativo de glicerol Stl1 e da primeira enzima da assimila{\c c}{\~a}o do glicerol, a glicerol quinase Gut1, com vista a aumentar o fluxo metab{\'o}lico de consumo do glicerol. Adicionalmente foi tamb{\'e}m sobrexpresso o fator de transcri{\c c}{\~a}o/ativador Pdc2 que controla v{\'a}rios genes, incluindo PDC1, PDC10 e THI10, com vista a promover o desvio do fluxo glicol{\'\i}tico para a fermenta{\c c}{\~a}o. Todas as constru{\c c}{\~o}es foram confirmadas por amplifica{\c c}{\~a}o de fragmentos alvo e sequenciamento de DNA. O desempenho final de cada mutante foi avaliado, n{\~a}o tendo sido obtido o efeito esperado. A taxa de crescimento foi tanto menor quanto mais modifica{\c c}{\~o}es gen{\'e}ticas, sugerindo uma diminui{\c c}{\~a}o de fitness proporcional. Em colabora{\c c}{\~a}o com o Laborat{\'o}rio Nacional de Ci{\^e}ncia e Tecnologia do Bioetanol (CTBE), o genoma da LBCM1105 foi sequenciado, montado, anotado e submetido a an{\'a}lises filogen{\'e}ticas multiloci. O genoma da W. anomalus LBCM1105 apresenta 12,78 Mb, com \%CG de 34,52, cobertura de 20,5x em profundidade e 90\% de extens{\~a}o e completude. Comparando com leveduras conhecidas, o genoma cont{\'e}m todas as sequ{\^e}ncias de prote{\'\i}nas relacionadas ao transporte e metabolismo de glicerol. O gene WaSTL1 foi isolado por PCR e clonado no vetor (pCev-G2-Km). O mutante S. cerevisiae BY4741 stl1Δ foi transformado com a constru{\c c}{\~a}o. A caracteriza{\c c}{\~a}o do mecanismo de transporte de glicerol confirmou (i) que WaStl1p tem uma elevada afinidade para o glicerol, Km = 0,96 {\textpm} 0,15 mM, (ii) que a cepa LBCM1105 apresenta al{\'e}m da afinidade uma elevada quantidade de transportador ativo, Vmax = 148,3 {\textpm} 10,16 μmol{\textperiodcentered}h-1{\textperiodcentered}g-1, e (iii) que o gene expresso heterologamente em S. cerevisiae stl1Δ complementa o mutante. O car{\'a}ter ativo do transporte foi confirmado pela sensibilidade ao protonion{\'o}foro carbonil cianeto m-clorofenilhidrazona, tanto na LBCM1105 como na S. cerevisiae stl1Δ WaSTL1. Futuros estudos das vias metab{\'o}licas de glicerol em leveduras ser{\~a}o necess{\'a}rios para que novas estrat{\'e}gias de engenharia gen{\'e}tica sejam mais eficientes.},
	url = {http://www.repositorio.ufop.br/handle/123456789/10813},
	author = {Aureliano Claret da Cunha}
}
@article {26701,
	title = {Alpha-Actinin-3 R577X Polymorphism Influences Muscle Damage and Hormonal Responses After a Soccer Game},
	journal = {JOURNAL OF STRENGTH AND CONDITIONING RESEARCH},
	volume = {33},
	number = {10},
	year = {2019},
	pages = {2655-2664},
	abstract = {
	The purpose of this study was to evaluate indicators of muscle damage and hormonal responses after soccer matches and its relation to alpha-actinin-3 (ACTN3) gene expression (XX vs. RR/RX), considering that the R allele produces alpha-actinin-3 and provides greater muscle strength and power. Thirty players (10 XX and 20 RR/RX) younger than 16 years were evaluated in this study. Blood samples were collected immediately before, after, 2, and 4 hours after the games to assess muscle damage (creatine kinase [CK] and alpha-actin) and hormonal responses (interleukin-6 [IL-6], cortisol, and testosterone). Postgame CK was higher as compared to the pregame values in both groups and it was also higher in the RR/RX (p\&nbsp;\&lt; 0.05) than in the XX. The concentrations of alpha-actin and IL-6 were similar for both groups and did not change over time. Testosterone was increased after the game only in the RR/RX group (p\&nbsp;\&lt; 0.05). Cortisol concentrations in group RR/RX were higher immediately after the game than before the game, and 2 and 4 hours after the game the concentration decreased (p\&nbsp;\&lt; 0.05). The RR and RX individuals presented higher markers of muscle microtrauma and hormonal stress, probably because they performed more speed and power actions during the game, which is a self-regulated activity. From the different responses presented by RR/RX and XX genotypes, we conclude that the genotypic profile should be taken into account when planning training workloads and recovery of athletes.

},
	url = {https://journals.lww.com/nsca-jscr/Abstract/2019/10000/Alpha_Actinin_3_R577X_Polymorphism_Influences.10.aspx},
	author = {Daniel B. COELHO and Eduardo M. PIMENTA and Rosse, Izinara C and Veneroso, Christiano and et. al.}
}
@article {26689,
	title = {Fractionation of sugarcane bagasse using hydrothermal and advanced oxidative pretreatments for bioethanol and biogas production in lignocellulose biorefineries},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {292},
	year = {2019},
	pages = {121963},
	abstract = {The fractionation of sugarcane bagasse (SB) by hydrothermal pretreatment (HP, autohydrolysis) followed by alkaline extraction (AE) and advanced oxidative pretreatment (AOP) for production of second-generation ethanol and biogas was investigated. The AOP of SB was optimized using a Doehlert design, varying the applied H2O2\&nbsp;load, liquid-to-solid ratio (LSR), and time. The responses evaluated were yield (Y), residual cellulose (RC), delignification (DE), and enzymatic conversion (EC). The AE of SB pretreated by HP led to 61.8\%\&nbsp;DE\&nbsp;(using 0.2 mol L-1\&nbsp;NaOH). This high lignin removal enabled substantial savings of H2O2\&nbsp;in the AOP. The optimized AOP conditions led to 78\%\&nbsp;Y, 82.2\%\&nbsp;RC, 42.7\%\&nbsp;DE, and 88.9\%\&nbsp;EC\&nbsp;(overall glucose yield of 60.9\%). Fermentation of the enzymatic hydrolysate with\&nbsp;Saccharomyces cerevisiae\&nbsp;yielded 190.8 Lethanol tonSB-1. Biogas production by anaerobic digestion of residual liquid streams of the pretreatment steps yielded 27.46 NLCH4 kgSB-1. An energy balance was estimated for the SB fractionation.},
	url = {https://doi.org/10.1016/j.biortech.2019.121963},
	author = {Gustavo Amaro Bittencourt and Elisa da Silva Barreto and Rogelio Lopes Brand{\~a}o and Bruno Eduardo Lobo Ba{\^e}ta and Leandro Vin{\'\i}cius Alves Gurgel}
}
@article {26687,
	title = {High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus.},
	journal = {JOURNAL OF INDUSTRIAL MICROBIOLOGY \& BIOTECHNOLOGY},
	volume = {46},
	number = {5},
	year = {2019},
	pages = {709{\textendash}723},
	url = {https://doi.org/10.1007/s10295-018-02119-5},
	author = {Aureliano Claret da Cunha and Lorena Soares Gomes and Fernanda Godoy-Santos and F{\'a}bio Faria-Oliveira and Jana{\'\i}na Aparecida Teixeira and Geraldo Magela Santos Sampaio and Maria Jos{\'e} Magalh{\~a}es Tr{\'o}pia and Ieso Miranda Castro, C{\^a}ndida Lucas and C{\^a}ndida Lucas and Rogelio Lopes Brand{\~a}o}
}
