@mastersthesis {26447,
	title = {An{\'a}lises de QTL e gen{\^o}mica comparativa para estudar mecanismos regulat{\'o}rios da H+-ATPase de membrana},
	year = {2021},
	type = {Tese de doutorado},
	abstract = {A H+ -ATPase {\'e} uma prote{\'\i}na presente na membrana citoplasm{\'a}tica de plantas e fungos e desempenha um papel essencial na gera{\c c}{\~a}o de um gradiente eletroqu{\'\i}mico de pr{\'o}tons, importante para a capta{\c c}{\~a}o de nutrientes e regula{\c c}{\~a}o do pH intracelular. A ativa{\c c}{\~a}o da H + -ATPase da membrana citoplasm{\'a}tica em Saccharomyces cerevisiae induzida por glicose {\'e} supostamente atribu{\'\i}da a um sinal de c{\'a}lcio intracelular correlacionado ao metabolismo do fosfatidilinositol. Em estudos anteriores do nosso grupo de pesquisa, foi observada diferen{\c c}a de fen{\'o}tipo entre as cepas S. cerevisiae BY4742 arg82Δ e PJ69 arg82Δ atrav{\'e}s do teste de atividade da H + -ATPase, indicando que o fen{\'o}tipo de maior ativa{\c c}{\~a}o da H + -ATPase est{\'a} relacionado a v{\'a}rios genes. Diante do exposto, o objetivo deste trabalho foi utilizar diferentes abordagens gen{\^o}micas para identificar novos componentes da regula{\c c}{\~a}o induzida por glicose da H + -ATPase. Para isso, foram realizados o sequenciamento do genoma segregante agrupado, mapeamento de QTLs e an{\'a}lises de Bioinform{\'a}tica para identificar o poss{\'\i}vel motivo da diferen{\c c}a de fen{\'o}tipo e, ou, novos componentes desta via de transdu{\c c}{\~a}o de sinal. Uma vez identificadas as variantes na an{\'a}lise de mapeamento de QTL foi desenvolvido o script SNPsInQTLselection.py para identificar as bases gen{\'e}ticas que podem estar envolvidas com o fen{\'o}tipo de maior atividade da H+ -ATPase. Ap{\'o}s a utiliza{\c c}{\~a}o desse script foram identificadas 42 variantes em 33 genes potencialmente envolvidos com o fen{\'o}tipo de interesse. Para prioriza{\c c}{\~a}o desses genes candidatos foi realizado an{\'a}lise de enriquecimento e interatoma que permitiram identificar 10 genes enriquecidos e envolvidos na via da telomerase e do fosfatidilinositol (STT4, PIK2, UGA2, EST1, MEC3, HEK2, TOP3, PSO2, STO1, FUR4). Cepas do background BY contendo essesrespectivos genes deletados (cole{\c c}{\~a}o EUROSCARF) foram utilizadas para teste de fen{\'o}tipo de acidifica{\c c}{\~a}o extracelular e sinaliza{\c c}{\~a}o de c{\'a}lcio induzida por glicose. Por meio desses testes, 4 (UGA2, FUR4, EST1 e STT4) dos 10 genes enriquecidos, apresentaram o fen{\'o}tipo de maior ativa{\c c}{\~a}o da H+ -ATPase, fen{\'o}tipo esse evidenciado nos testes de acidifica{\c c}{\~a}o extracelular e sinal de c{\'a}lcio. Destes 4 genes, vale destacar o STT4 visto que, de acordo com a fun{\c c}{\~a}o descrita para esse gene na literatura o fosfatidilinositol-4-fosfato pode exercer um papel na regula{\c c}{\~a}o da via de ativa{\c c}{\~a}o de H + -ATPase, controlando a atividade da fosfolipase C. Tendo em vista uma rela{\c c}{\~a}o positiva entre atividade da H + - ATPase e desempenho fermentativo, estudos futuros podem ser feitos com os genes/alelos identificados neste trabalho, com aplica{\c c}{\~a}o em cepas de leveduras industriais 8 visando a melhoria na efici{\^e}ncia de processos fermentativos. Os genes (UGA2, FUR4, EST1 e STT4) merecem investiga{\c c}{\~a}o detalhada para verifica{\c c}{\~a}o do poss{\'\i}vel envolvimento na via de sinaliza{\c c}{\~a}o da H+ -ATPase. Esse trabalho apresenta pela primeira vez uma estrat{\'e}gia de Bioinform{\'a}tica para identificar genes candidatos quando o resultado do mapeamento de QTL n{\~a}o for significativo.},
	url = {	http://www.repositorio.ufop.br/jspui/handle/123456789/13846},
	author = {Patr{\'\i}cia Gon{\c c}alves Prates Barbosa}
}
@article {26445,
	title = {On-site Produced Enzyme Cocktails for Saccharification and Ethanol Production from Sugarcane Bagasse Fractionated by Hydrothermal and Alkaline Pretreatments},
	journal = {Waste and Biomass Valorization},
	volume = {13},
	year = {2021},
	pages = {95-106},
	abstract = {Enzymatic blends produced by fungal monocultures and consortia cultured in solid-state fermentation (SSF), using sugarcane bagasse (SB) and wheat bran as substrates (1:1, w/w), were evaluated for saccharification of sugarcane bagasse pretreated by autohydrolysis (hydrothermal pretreatment{\textemdash}HP) and alkaline delignification (HP-Soda). The highest glucose releases were obtained after saccharifications of SB pretreated by HP using enzyme cocktails produced by\&nbsp;Aspergillus niger\&nbsp;and by the consortium among\&nbsp;A. fumigatus,\&nbsp;Ganoderma lucidum\&nbsp;and\&nbsp;Trametes versicolor, with 10.8 and 9.8\&nbsp;g L-1, respectively. For SB pretreated by HP-Soda, the hydrolysate 10 (extract from\&nbsp;A. niger, G. lucidum\&nbsp;and\&nbsp;Pleurotus ostreatus\&nbsp;consortium) achieved maximal glucose concentration (11.92\&nbsp;g L-1). After alcoholic fermentation of the hydrolysates, the greatest ethanol yield in relation to the maximum theoretical yield (60.8\%) was obtained in the fermentation of hydrolysate 1 (A. niger) obtained from SB pretreated by HP-Soda. These results demonstrated that on-site produced enzyme cocktails can be applied for saccharification of pretreated sugarcane bagasse and also contribute to cost reduction of bioconversion processes.},
	url = {https://doi.org/10.1007/s12649-021-01499-7},
	author = {Patr{\'\i}sia de Oliveira Rodrigues and Elisa da Silva Barreto and Rog{\'e}lio Lopes Brand{\~a}o and Leandro Vin{\'\i}cius Alves Gurgel and Daniel Pasquini and Milla Alves Baffi}
}
@article {26694,
	title = {Genetic Diversity and Population Genetic Structure of a Guzer{\'a} (Bos indicus) Meta-Population},
	journal = {Animals},
	volume = {11},
	number = {4},
	year = {2021},
	abstract = {The Brazilian Guzer{\'a} population originated from a few founders introduced from India. These animals adapted well to the harsh environments in Brazil, were selected for beef, milk, or dual-purpose (beef and milk), and were extensively used to produce crossbred animals. Here, the impact of these historical events with regard to the population structure and genetic diversity in a Guzer{\'a} meta-population was evaluated. DNA samples of 744 animals (one dairy, nine dual-purpose, and five beef herds) were genotyped for 21 microsatellite loci. Ho, He, PIC, Fis, Fit, and Fst\&nbsp;estimates were obtained considering either farms or lineages as subpopulations. Mean Ho (0.73) and PIC (0.75) suggest that genetic diversity was efficiently conserved. Fit, Fis\&nbsp;and Fst\&nbsp;values (95\% CI) pointed to a low fixation index, and large genetic diversity: Fit\&nbsp;(Farms = 0.021-0.100; lineages = 0.021-0.100), Fis\&nbsp;(Farms = -0.007-0.076; lineages = -0.014-0.070), and Fst\&nbsp;(Farms = 0.0237-0.032; lineages = 0.029-0.038). The dual-purpose herds/selection lines are the most uniform subpopulation, while the beef one preserved larger amounts of genetic diversity among herds. In addition, the dairy herd showed to be genetically distant from other herds. Taken together, these results suggest that this Guzer{\'a} meta-population has high genetic diversity, a low degree of population subdivision, and a low inbreeding level.},
	url = {https://pubmed.ncbi.nlm.nih.gov/33919992/},
	author = {Maria Gabriela C D Peixoto and Maria Raquel S Carvalho and Andrea A Egito and Raphael S Steinberg and Frank {\^A}ngelo T Bruneli and Marco Ant{\^o}nio Machado and Fernanda C Santos and Izinara C. Rosse and Pablo Augusto S Fonseca}
}
@article {26698,
	title = {Identification and in silico characterization of structural and functional impacts of genetic variants in milk protein genes in the Zebu breeds Guzerat and Gyr},
	journal = { TROPICAL ANIMAL HEALTH AND PRODUCTION},
	volume = {53},
	year = {2021},
	pages = {254, 2021},
	abstract = {Whole genome sequencing of bovine breeds has allowed identification of genetic variants in milk protein genes. However, functional repercussion of such variants at a molecular level has seldom been investigated. Here, the results of a multistep Bioinformatic analysis for functional characterization of recently identified genetic variants in Brazilian Gyr and Guzerat breeds is described, including predicted effects on the following: (i) evolutionary conserved nucleotide positions/regions; (ii) protein function, stability, and interactions; (iii) splicing, branching, and miRNA binding sites; (iv) promoters and transcription factor binding sites; and (v) collocation with QTL. Seventy-one genetic variants were identified in the caseins (CSN1S1,\&nbsp;CSN2,\&nbsp;CSN1S2, and\&nbsp;CSN3),\&nbsp;LALBA,\&nbsp;LGB, and\&nbsp;LTF\&nbsp;genes. Eleven potentially regulatory variants and two missense mutations were identified. LALBA Ile60Val was predicted to affect protein stability and flexibility, by reducing the number the disulfide bonds established. LTF Thr546Asn is predicted to generate steric clashes, which could mildly affect iron coordination. In addition, LALBA Ile60Val and LTF Thr546Asn affect exonic splicing enhancers and silencers. Consequently, both mutations have the potential of affecting immune response at individual level, not only in the mammary gland. Although laborious, this multistep procedure for classifying variants allowed the identification of potentially functional variants for milk protein genes.},
	url = {https://doi.org/10.1007/s11250-021-02970-2},
	author = {Carolina Guimar{\~a}es Ramos Matosinho and Izinara Cruz Rosse and Pablo Augusto Souza Fonseca and Francislon Silva de Oliveira and Fausto Gon{\c c}alves dos Santos and Fl{\'a}vio Marcos Gomes Ara{\'u}jo and Anna Christina de Matos Salim and Beatriz Cordenonsi Lopes and et. al.}
}
@article {26697,
	title = {Is lin28a polymorphism associated with endurance performance in soccer players?},
	journal = {SPORT SCIENCES FOR HEALTH },
	year = {2021},
	abstract = {
	Purpose



	The aim of this study was to investigate the association of rs6598964 (A \&gt; G), a molecular marker located in the\&nbsp;LIN28A\&nbsp;gene, with the performance of Brazilian soccer players using the VO2max predicted by performance in the Yo-Yo test as the phenotype.



	Methods



	The study sample comprised 227 male players on a team in the first division of Brazilian soccer distributed in the following categories: U15 (n = 67, VO2max = 52.75 {\textpm} 4.74\&nbsp;ml/kg/min), U17 (n = 43, VO2max = 54.37 {\textpm} 5.47\&nbsp;ml/kg/min), U20 (n = 79, VO2max = 54.97 {\textpm} 5.13\&nbsp;ml/kg/min), and Professional (n = 38, VO2max = 55.84 {\textpm} 4.37\&nbsp;ml/kg/min). Genotype models (codominance, A-recessive, A-dominant and overdominance models) were tested using Kruskal{\textendash}Wallis and Mann{\textendash}Whitney tests with Bonferroni correction for multiple comparisons tests.



	Results



	Significantly higher predicted VO2max was observed in individuals with the A/A genotype (VO2max = 62.12 {\textpm} 3.97\&nbsp;mL/kg/min) compared to both the A/G (53.44 {\textpm} 8.88\&nbsp;mL/kg/min) and G/G (52.44 {\textpm} 6.11\&nbsp;mL/kg/min) genotypes (p \&lt; 0.001). Model comparisons suggested the differences in predicted VO2max were best explained by the A-recessive model.



	Conclusion



	This study is the first to associate the\&nbsp;LIN28A\&nbsp;polymorphism with endurance performance in soccer players. However, further studies are needed to confirm the associations described here and to investigate how\&nbsp;LIN28A\&nbsp;interacts with other genes related to athletic performance.

},
	url = {https://doi.org/10.1007/s11332-021-00812-0},
	author = {T. Kanope and E. M. Pimenta and C. Veneroso and D. Coelho and L. F. Oliveira and E. Silami-Garcia and R. F. Morandi and M. R. S. Carvalho and I. C. Rosse}
}
@article {26681,
	title = {Moniliophthora perniciosa, the causal agent of cacao Witches\&$\#$39; Broom Disease is killed in vitro by Saccharomyces cerevisiae and Wickerhamomyces anomalus yeasts. },
	journal = { Frontiers in Microbiology},
	volume = {1},
	year = {2021},
	pages = {1-8},
	abstract = {Cacao plantations from South America have been afflicted with the severe fungal disease known as\&nbsp;Witches{\textquoteright} Broom Disease\&nbsp;(WBD), caused by the basidiomycete\&nbsp;Moniliophthora perniciosa. Yeasts are increasingly recognized as good fungal biocides, although their application is still mostly restricted to the postharvest control of plant and fruit decay. Their possible utilization in the field, in a preharvest phase, is nevertheless promising, particularly if the strains are locally adapted and evolved and if they belong to species considered safe for man and the environment. In this work, a group of yeast strains originating from sugarcane-based fermentative processes in Brazil, the cacao-producing country where the disease is most severe, were tested for their ability to antagonize\&nbsp;M. perniciosa in vitro.\&nbsp;Wickerhamomyces anomalus\&nbsp;LBCM1105 and\&nbsp;Saccharomyces cerevisiae\&nbsp;strains LBCM1112 from spontaneous fermentations used to produce\&nbsp;cacha{\c c}a, and PE2 widely used in Brazil in the industrial production of bioethanol, efficiently antagonized six strains of\&nbsp;M. perniciosa, originating from several South American countries. The two fastest growing fungal strains, both originating from Brazil, were further used to assess the mechanisms underlying the yeasts{\textquoteright} antagonism. Yeasts were able to inhibit fungal growth and kill the fungus at three different temperatures, under starvation, at different culture stages, or using an inoculum from old yeast cultures. Moreover, SEM analysis revealed that\&nbsp;W. anomalus\&nbsp;and\&nbsp;S. cerevisiae\&nbsp;PE2 cluster and adhere to the hyphae, push their surface, and fuse to them, ultimately draining the cells. This behavior concurs with that classified as necrotrophic parasitism/mycoparasitism. In particular,\&nbsp;W. anomalus\&nbsp;within the adhered clusters appear to be ligated to each other through roundish groups of fimbriae-like structures filled with bundles of microtubule-sized formations, which appear to close after cells detach, leaving a scar. SEM also revealed the formation of tube-like structures apparently connecting yeast to hypha. This evidence suggests\&nbsp;W. anomalus\&nbsp;cells form a network of yeast cells connecting with each other and with hyphae, supporting a possible cooperative collective killing and feeding strategy. The present results provide an initial step toward the formulation of a new eco-friendly and effective alternative for controlling cacao WBD using live yeast biocides.},
	url = {https://www.frontiersin.org/articles/10.3389/fmicb.2021.706675/full},
	author = {Pedro Ferraz and Rogelio Lopes Brand{\~a}o and Fernanda C{\'a}ssio and C{\^a}ndida Lucas}
}
@article {26693,
	title = {Polymorphism of the angiotensin converting enzyme gene (ACE-I/D) differentiates the aerobic and speed performance of football players},
	journal = {THE JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS},
	volume = {62},
	number = {2},
	year = {2021},
	pages = {192-198},
	abstract = {The aim of this study was to evaluate the distribution of ACE-I/D polymorphisms on Brazilian football players performance in aerobic capacity, strength and speed tests.METHODS: The participants in this study were 212 Brazilian first division male football players genotyped in DD, ID or II. Genotyping of DNA from leucocytes was performed using polymerase chain reaction and restriction fragment length polymorphism methods. We evaluated speed using a 30-meter sprint test with speed measured at 10 meters (V10), 20 meters (V20), and 30 meters (V30); muscular strength using counter-movement-jump and squat jump tests; and aerobic endurance using the Yo-Yo endurance test. The athletes were ranked in ascending order according to their performance in each test and divided into quartiles: first quartile (0-25\%, weak), second (25-50\%, normal), third (50-75\%, good), and fourth (75-100\%, excellent); these were clustered according to genotype frequency.RESULTS: We identified significant differences in the V20 test values and in the aerobic capacity test. Higher frequencies of the\&nbsp;ACE-DD\&nbsp;genotype were observed in the excellent performance group in the V20. In the aerobic capacity test, higher frequencies of the\&nbsp;ACE-II\&nbsp;genotype were observed in excellent and good performance groups.CONCLUSIONS: Players with higher performance in anaerobic and aerobic tests are\&nbsp;ACE-DD\&nbsp;and\&nbsp;ACE-II\&nbsp;genotypes, respectively.},
	url = {https://www.minervamedica.it/en/journals/sports-med-physical-fitness/article.php?cod=R40Y2022N02A0192},
	author = {Daniel B. COELHO and Eduardo M. PIMENTA and Izinara C. Rosse and Emerson C. de OLIVEIRA and Lenice K. BECKER and Jo{\~a}o B. FERREIRA-J{\'U}NIOR and Lilian M. LOPES and Maria R. CARVALHO and Emerson SILAMI-GARCIA}
}
@article {26699,
	title = {Putative Causal Variant on Vlgr1 for the Epileptic Phenotype in the Model Wistar Audiogenic Rat. },
	journal = {Frontiers in Neurology},
	year = {2021},
	abstract = {
	Wistar Audiogenic Rat is an epilepsy model whose animals are predisposed to develop seizures induced by acoustic stimulation. This model was developed by selective reproduction and presents a consistent genetic profile due to the several generations of inbreeding. In this study, we performed an analysis of WAR RNA-Seq data, aiming identified at genetic variants that may be involved in the epileptic phenotype. Seventeen thousand eighty-five predicted variants were identified as unique to the WAR model, of which 15,915 variants are SNPs and 1,170 INDELs. We filter the predicted variants by pre-established criteria and selected five for validation by Sanger sequencing. The genetic variant c.14198T\&gt;C in the\&nbsp;Vlgr1\&nbsp;gene was confirmed in the WAR model.\&nbsp;Vlgr1\&nbsp;encodes an adhesion receptor that is involved in the myelination process, in the development of stereocilia of the inner ear, and was already associated with the audiogenic seizures presented by the mice Frings. The transcriptional quantification of\&nbsp;Vlgr1\&nbsp;revealed the downregulation this gene in the corpus quadrigeminum of WAR, and the protein modeling predicted that the mutated residue alters the structure of a domain of the VLGR1 receptor. We believe that\&nbsp;Vlgr1\&nbsp;gene may be related to the predisposition of WAR to seizures and suggest the mutation\&nbsp;Vlgr1/Q4695R as putative causal variant, and the first molecular marker of the WAR strain.

},
	url = {https://doi.org/10.3389/fneur.2021.647859},
	author = {Samara Damasceno and Pablo Augusto de Souza Fonseca and Izinara Cruz Rosse and M{\'a}rcio Fl{\'a}vio Dutra Moraes and Jos{\'e} Ant{\^o}nio Cortes de Oliveira and Norberto Garcia-Cairasco and Ana L{\'u}cia Brunialti Godard}
}
